Data for the Constructed Mutant: YBFTd

General Information

Name of the MutantYBFTd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size747 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:

Mutant Construction for YBFTd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ggaagct tag) ybfT-F: ggaagctTACAAGCTAAGAAAGATGCC
Reverse Primer:(ttgga tag) ybfT-R: ttggaTCCCGCCTGGCGGATGAGAT
Length of the Cloned Region: 71 to 372 Length: 302
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBFTd