Data for the Constructed Mutant: YBFMd

General Information

Name of the MutantYBFMd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size486 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:

Mutant Construction for YBFMd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ggaagctt tag) YBFM-F: ggaagcttGTAGGATTGCCGATTC
Reverse Primer:(ttggatcc tag) YBFM-R: ttggatccGGCTTTTCTGCCAATC
Length of the Cloned Region: 73 to 222 Length: 150
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBFMd