Data for the Constructed Mutant: YBFId

General Information

Name of the MutantYBFId (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size825 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBFId (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybfI-H: aagaagcttAAGCCTACCGCTTCAAGGGA
Reverse Primer:(cgcagatct tag) ybfI-B: cgcagatctGGAGAAATAAGGGAGATGCCC
Length of the Cloned Region: 50 to 330 Length: 281
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBFId