Data for the Constructed Mutant: YBFId
General Information
Name of the Mutant
YBFId (disruption)
Host
168
Used Vector
pMutin2
Used Enzyme Sites
HindIII;,BamHI;,BglII
Gene Size
825 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
yes
Mutant Submission to NIG:
yes
Correspondence:
Mutant Construction for YBFId (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
aagaagctt
tag)
ybfI-H:
aagaagcttAAGCCTACCGCTTCAAGGGA
Reverse Primer:
(
cgcagatct
tag)
ybfI-B:
cgcagatctGGAGAAATAAGGGAGATGCCC
Length of the Cloned Region: 50 to 330 Length: 281
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR: yes
Primer sets used for the check
No.
Primer set
Length of PCR product
1
Forward Primer
ybfI-5
GAGCATGCTCGTAACTGTCA
Reverse Primer
ybfG-3
GTTGAATCTGGCGAATTCTTGAATC
11.3k
Digestion of the PCR product: yes
Enzyme used: BanIII
Figure
Comment: ;.
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YBFId