Data for the Constructed Mutant: YBFHd

General Information

Name of the MutantYBFHd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size918 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBFHd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybfH-H: aagaagcttCTGATTTGGGGGACGACC
Reverse Primer:(cgcagatct tag) ybfH-B: cgcagatctCCCGATCAGTGCCGAGATAA
Length of the Cloned Region: 52 to 405 Length: 354
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBFHd