Data for the Constructed Mutant: YBFGd

General Information

Name of the MutantYBFGd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size2196 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBFGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybfG-H: aagaagcttCTACGGTCTGACTCGGGCGC
Reverse Primer:(cgcagatct tag) ybfG-B: cgcagatctACACCAGCTTAAAAGCGCTC
Length of the Cloned Region: 111 to 430 Length: 320
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBFGd