Data for the Constructed Mutant: YBFFd

General Information

Name of the MutantYBFFd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size909 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBFFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybfF-H: aagaagcttAAGAAGCTTATTCTCTGACACTCAACCAG
Reverse Primer:(cgcagatct tag) ybfF-B: cgcagatctATTATCAGAGGCAGTATGTT
Length of the Cloned Region: 913 to 483 Length: 431
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBFFd