Data for the Constructed Mutant: YBFBd

General Information

Name of the MutantYBFBd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size1248 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBFBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybfB-H: aagaagcttAAGAAGCTTCGTGGATCATCGTCTCCGTC
Reverse Primer:(cgcagatct tag) ybyB-B: cgcagatctCATTCGATGCTCCGCCCACT
Length of the Cloned Region: -1248 to -1248 Length: 1
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBFBd