Data for the Constructed Mutant: YBECd

General Information

Name of the MutantYBECd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size1617 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBECd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybeC-H: aagaagcttGCGGCTCAAATAGCAGGAC
Reverse Primer:(cgcagatct tag) ybeC-B: cgcagatctGTCCAGTAGTTCAGCAGA
Length of the Cloned Region: 97 to 458 Length: 362
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBECd