Data for the Constructed Mutant: YBDNd

General Information

Name of the MutantYBDNd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size855 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBDNd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybdN-H: aagaagcttGAACGGCGTTATCTTAAATC
Reverse Primer:(cgcagatct tag) ybdN-B: cgcagatctCTATATTCTCTCCCTCAATG
Length of the Cloned Region: 992 to 1275 Length: 284
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBDNd