Data for the Constructed Mutant: YBDLdd

General Information

Name of the MutantYBDLdd (deletion (double-cross))
Host168
Used VectorpMutin1
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size192 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YBDLdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(gtctcgag tag) ybdL-X1:gtctcgagGCATTTGGCGAGTGATCA
Reverse Primer:(gtggatcc tag) ybdL-BA:gtggatccGGTGTCTGCGATATCGGT
Downstream:
Forward Primer:(gtaagctt tag) ybdL-HD:gtaagcttACAAACGCCGGAGCTACA
Reverse Primer:(gtctcgag tag) ybdL-X2: gtctcgagTGCAGACATGGCATTAAA
Linearization:
Length of the Deletion: 70 to 167 Length: 98


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YBDLdd