Data for the Constructed Mutant: YBDGd

General Information

Name of the MutantYBDGd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size888 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBDGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybdG-H: aagaagcttAAAACATTATGGAAAGTCCT
Reverse Primer:(cgcagatct tag) ybdG-B: cgcagatctCCCGAACCGGACATAAACAC
Length of the Cloned Region: 4 to 221 Length: 218
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBDGd