Data for the Constructed Mutant: YBDEd

General Information

Name of the MutantYBDEd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size423 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBDEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybdE-H: aagaagcttAAAGATGGACAAATGTTGA
Reverse Primer:(cgcagatct tag) ybdE-B: cgcagatctGGACATGTACTCCGATTACT
Length of the Cloned Region: 4 to 202 Length: 199
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBDEd