Data for the Constructed Mutant: YBDBd

General Information

Name of the MutantYBDBd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size1344 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBDBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybdB-H: aagaagcttTATTAGGCCGATCCATAAT
Reverse Primer:(cgcagatct tag) ybdB-B: cgcagatctACCTGACGGCTCGTA
Length of the Cloned Region: 326 to 641 Length: 316
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBDBd