Data for the Constructed Mutant: YBCTdd

General Information

Name of the MutantYBCTdd (deletion (double-cross))
Host168
Used VectorpMutin1
Used Enzyme SitesHindIII;,BamHI;,XhoI
Gene Size933 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YBCTdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(gtctcgag tag) ybcT-X1:gtctcgagGGAAGCACGTCATAAATGG
Reverse Primer:(gtggatcc tag) ybcT-BA:gtggatccAGCATACCAGATACTCCG
Downstream:
Forward Primer:(atgaagctt tag) ybcT-H:atgaagcttCAGCTTTAGCGATGATCC
Reverse Primer:(gtctcgag tag) ybcT-X2: gtctcgagAACTGCCACAGCGGCATAG
Linearization:
Length of the Deletion: 128 to 134 Length: 7


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YBCTdd