Data for the Constructed Mutant: YBCFd

General Information

Name of the MutantYBCFd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII;,BamHI;,BglII
Gene Size525 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YBCFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) ybcF-H: aagaagcttACGGATATTGAAAACGG
Reverse Primer:(gtagatct tag) ybcF-B: gtagatctCAGGCTTGCAATTTTGAAAC
Length of the Cloned Region: 37 to 316 Length: 280
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBCFd