Data for the Constructed Mutant: YAZAdd

General Information

Name of the MutantYAZAdd (deletion (double-cross))
Host168
Used VectorpMutin2
Used Enzyme SitesEcoRI-XhoI & XhoI-BamHI
Gene Size297 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YAZAdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcg tag) yazA-UF:ctcctcgAGAAAGACTGCATGATATGG
Reverse Primer:(ggaggatc tag) yazA-UR:ggaggatcCGTAGAAGAAATGGTTATTTG
Downstream:
Forward Primer:(gaagaatt tag) yazA-DF:gaagaattCTTTATATAGAAGAAAAGCG
Reverse Primer:(ctcctcg tag) yazA-DR: ctcctcgAGGGCTTCAAGCTCCTTCTTC
Linearization:
Length of the Deletion: 28 to 235 Length: 208


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YAZAdd