Data for the Constructed Mutant: ACOLd

General Information

Name of the MutantACOLd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesEcoRI and BamHI
Gene Size1374 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for ACOLd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccggaattc tag) jHEF: gccggaattcATGCGGCTGCGGTTTCC
Reverse Primer:(gcgcggatcc tag) jHBR: gcgcggatccACAAGCTGACTGACAACC
Length of the Cloned Region: 38 to 278 Length: 241
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of ACOLd