Data for the Constructed Mutant: YXLGd

General Information

Name of the MutantYXLGd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size777 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXLGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxlGH: GCCGaagcttTATTGCAGAAGGAGTGGCTG
Reverse Primer:(agatct tag) yxlGBG: GCGCagatctATAATGGGCTGCCGTCACAG
Length of the Cloned Region: 20 to 342 Length: 323
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXLGd