Data for the Constructed Mutant: YXLFd

General Information

Name of the MutantYXLFd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size885 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXLFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxlFH: GCCGaagcttATCGTACAGGCACCATGAAG
Reverse Primer:(agatct tag) yxlFBG: GCGCagatctACAAATTCCAGCATCTCGCC
Length of the Cloned Region: 27 to 344 Length: 318
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXLFd