Data for the Constructed Mutant: YXKOd

General Information

Name of the MutantYXKOd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size828 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXKOd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxkOH: GCCGaagcttGTCATCGGTACATCAGAAAAC
Reverse Primer:(agatct tag) yxkOBG: GCGCagatctTCTGCAATTCGTTCACGGGC
Length of the Cloned Region: 175 to 496 Length: 322
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXKOd