Data for the Constructed Mutant: YFHRd

General Information

Name of the MutantYFHRd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesEcoRI and BamHI
Gene Size750 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YFHRd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccggaattc tag) EYFHR: gccggaattcAATGTGCACTCGTAACAGG
Reverse Primer:(cgcggatcc tag) BYFHR: cgcggatccCATTAATGTTCATCGTCCAG
Length of the Cloned Region: 14 to 340 Length: 327
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFHRd