Data for the Constructed Mutant: YFINd

General Information

Name of the MutantYFINd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1155 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:

Mutant Construction for YFINd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) iNHF: gccgaagcttCCATTTGCGGCATTGAGC
Reverse Primer:(gcgcggatcc tag) iNBR: gcgcggatccGCTTTTCTGACGCTTTGC
Length of the Cloned Region: 17 to 262 Length: 246
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFINd