Data for the Constructed Mutant: YFIJd

General Information

Name of the MutantYFIJd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1200 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YFIJd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) IJ-1H: gccgaagcttGTTCACCTTGATGGTGAG
Reverse Primer:(gcgcggatcc tag) IJ-2B: gcgcggatccAGACAGACCATGCTGTTC
Length of the Cloned Region: 27 to 307 Length: 281
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFIJd