Data for the Constructed Mutant: YEAAd

General Information

Name of the MutantYEAAd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size987 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YEAAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:( tag) : ACACGATCAGATATTAA
Reverse Primer:( tag) : TGCCAGCAGTTGATAGC
Length of the Cloned Region: 991 to 991 Length: 1
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YEAAd