Data for the Constructed Mutant: YDILd

General Information

Name of the MutantYDILd (disruption)
Host
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size732 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YDILd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) : gccgaagcttTTCGGTTATGCCGGAGG
Reverse Primer:(cgcggatcc tag) : cgcggatccTAATATAGGCCCGACGA
Length of the Cloned Region: 85 to 426 Length: 342
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YDILd