Data for the Constructed Mutant: YDHLd

General Information

Name of the MutantYDHLd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1275 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YDHLd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) : gccgaagcttACATTTCCATTGTTTCA
Reverse Primer:(cgcggatcc tag) : cgcggatccTGAACTCGCGATTTTCA
Length of the Cloned Region: 212 to 723 Length: 512
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDHLd