Data for the Constructed Mutant: YDHDd

General Information

Name of the MutantYDHDd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1317 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YDHDd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) : gccgaagcttCTCCGAAACCCAGACCT
Reverse Primer:(cgcggatcc tag) : cgcggatccAGGAACAGCTATCGTCA
Length of the Cloned Region: 322 to 735 Length: 414
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDHDd