Data for the Constructed Mutant: YDHCd

General Information

Name of the MutantYDHCd (disruption)
Host
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size672 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YDHCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) : gccgaagcttAAAAAAATGATCTTCAA
Reverse Primer:(cgcggatcc tag) : cgcggatccTCTGTTATGGCTGAAAT
Length of the Cloned Region: 61 to 450 Length: 390
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YDHCd