Data for the Constructed Mutant: YDGGd

General Information

Name of the MutantYDGGd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size456 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YDGGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydgG-F: gccgaagcttAACAAAAGCTATGTCAGG
Reverse Primer:(cgcggatcc tag) ydgG-R: cgcggatccACATGGCCAGGCTGCCGG
Length of the Cloned Region: 8 to 181 Length: 174
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDGGd