Data for the Constructed Mutant: YDFOd

General Information

Name of the MutantYDFOd (disruption)
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size936 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YDFOd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydfO-F: gccgaagcttGGTCACCCTCAAGAGAACACAG
Reverse Primer:(cgcggatcc tag) ydfO-R: cgcggatccGCAACATCGGGCGTTACTTCTC
Length of the Cloned Region: 46 to 446 Length: 401
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YDFOd