Data for the Constructed Mutant: YDFLd

General Information

Name of the MutantYDFLd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size810 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YDFLd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydfL-F: gccgaagcttTGTCAAAGTTATTCAATG
Reverse Primer:(cgcggatcc tag) ydfL-R: cgcggatccTGATTGGCAGGCCTAGGG
Length of the Cloned Region: 29 to 196 Length: 168
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDFLd