Data for the Constructed Mutant: YDFDd

General Information

Name of the MutantYDFDd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1446 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YDFDd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydfD-F: gccgaagcttAAAGCTCCCTAAGTACAG
Reverse Primer:(cgcggatcc tag) ydfD-R: cgcggatccTCTTAGCCATCAGTGTCC
Length of the Cloned Region: 30 to 262 Length: 233
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDFDd