Data for the Constructed Mutant: YDFAd

General Information

Name of the MutantYDFAd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1305 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YDFAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydfA-F: gccgaagcttCTGTGGAGGCGCAGTTTTAG
Reverse Primer:(cgcggatc tag) ydfA-R: cgcggatcCAGGTACGGGACAACCATTC
Length of the Cloned Region: 93 to 561 Length: 469
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDFAd