Data for the Constructed Mutant: YDEOd

General Information

Name of the MutantYDEOd (disruption)
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size870 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YDEOd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydeO-F: gccgaagcttCATCATGGTTATCATTGG
Reverse Primer:(cgcggatcc tag) ydeO-R: cgcggatccCTTATATCCTAACCAGAC
Length of the Cloned Region: 54 to 234 Length: 181
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YDEOd