Data for the Constructed Mutant: YDDRd

General Information

Name of the MutantYDDRd (disruption)
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size762 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YDDRd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) yddR-F : gccgaagcttGAAATGCAACAATTGTTG
Reverse Primer:(cgcggatcc tag) yddR-R: cgcggatccGTGATCTAGGTGTAGATG
Length of the Cloned Region: 20 to 219 Length: 200
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YDDRd