Data for the Constructed Mutant: YDCFdd

General Information

Name of the MutantYDCFdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII-EcoRI & EcoRI-BamHI
Gene Size291 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YDCFdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(cggtgagaattc tag) ydcF-1F:cggtgagaattcTTCTGCATTCCCCGTCTG
Reverse Primer:(cgcggatcc tag) ydcF-1R:cgcggatccCAGAAGCAATGTTCCTTCCG
Downstream:
Forward Primer:(gccgaagctt tag) ydcF-2F:gccgaagcttGTCCATCTTTACGGAGGAG
Reverse Primer:(cggtgagaattc tag) ydcF-2R: cggtgagaattcCGCTGCCAACTTTGCGTG
Linearization:
Length of the Deletion: 26 to 265 Length: 240


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YDCFdd