Data for the Constructed Mutant: YDBNdd

General Information

Name of the MutantYDBNdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII-EcoRI & EcoRI-BamHI
Gene Size177 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YDBNdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(cggtgagaattc tag) ydbN-1F:cggtgagaattcACGCAACAATATCTGTTC
Reverse Primer:(cgcggatcc tag) ydbN-1R:cgcggatccTCACTTTCTAACTCCGTCAG
Downstream:
Forward Primer:(gccgaagctt tag) ydbN-2F:gccgaagcttATCTGCAAGAGCAAAACAAT
Reverse Primer:(cggtgagaattc tag) ydbN-2R: cggtgagaattcGGTGGAACTGCTTCAAGG
Linearization:
Length of the Deletion: 5 to 154 Length: 150


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YDBNdd