Data for the Constructed Mutant: YDBFd

General Information

Name of the MutantYDBFd (disruption)
Host168
Used VectorpMutinT3
Used Enzyme Sites
Gene Size1605 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YDBFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) ydbF-F: cgaggcagatctGCTCTCAATCCGTTGGAAAATCACG
Reverse Primer:(cgaggcagatctctcgag tag) ydbF-R: cgaggcagatctctcgagGCTTGTGCTGACAGGATGC
Length of the Cloned Region: 15 to 318 Length: 304
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YDBFd