Data for the Constructed Mutant: YDBBd

General Information

Name of the MutantYDBBd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size339 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:

Mutant Construction for YDBBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydbB-F: gccgaagcttTTTCGCTAATGTCCAAGATG
Reverse Primer:(cgcggatcc tag) ydbB-R: cgcggatccGATCAGCAGCTCCCCTTC
Length of the Cloned Region: -8 to 183 Length: 192
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDBBd