Data for the Constructed Mutant: YDASdd

General Information

Name of the MutantYDASdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII-EcoRI & EcoRI-BamHI
Gene Size255 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YDASdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(cggtgagaattc tag) ydaS-1F:cggtgagaattcCGATGAAGGATTATCCTG
Reverse Primer:(cgcggatcc tag) ydaS-1R:cgcggatccAAGCTAAGCATCATGATCAC
Downstream:
Forward Primer:(gccgaagctt tag) ydaS-2F:gccgaagcttGGCTGCGGAAGGAAGCCTAG
Reverse Primer:(cggtgagaattc tag) ydaS-2R: cggtgagaattcGCAATACCGAGTTTGGCG
Linearization:
Length of the Deletion: 12 to 238 Length: 227


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YDASdd