Data for the Constructed Mutant: YDAQdd

General Information

Name of the MutantYDAQdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII-EcoRI & EcoRI-BamHI
Gene Size288 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YDAQdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(cggtgagaattc tag) ydaQ-1F:cggtgagaattcTTGAGTACGAGCAGCAGGTC
Reverse Primer:(cgcggatcc tag) ydaQ-1R:cgcggatccATAAGAATAAGCATGCAAGG
Downstream:
Forward Primer:(gccgaagctt tag) ydaQ-2F:gccgaagcttAAAACAGAATAAATACTGAC
Reverse Primer:(cggtgagaattc tag) ydaQ-2R: cggtgagaattcTTGTCAGCCCTATGTCTGCG
Linearization:
Length of the Deletion: 14 to 290 Length: 277


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YDAQdd