Data for the Constructed Mutant: YDAMd

General Information

Name of the MutantYDAMd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1260 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:

Mutant Construction for YDAMd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydaM-F: gccgaagcttACATGTTCCTCATGCAGG
Reverse Primer:(cgcggatcc tag) ydaM-R: cgcggatccATCTCCAGCCGGTCCTTC
Length of the Cloned Region: 59 to 248 Length: 190
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDAMd