Data for the Constructed Mutant: YDAKd

General Information

Name of the MutantYDAKd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size849 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:

Mutant Construction for YDAKd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydaK-F: gccgaagcttTTCAGGGCTCATCGCGG
Reverse Primer:(cgcggatcc tag) ydaK-R: cgcggatccAGCGATGTCTGTCCAGTC
Length of the Cloned Region: 45 to 248 Length: 204
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDAKd