Data for the Constructed Mutant: YDAId

General Information

Name of the MutantYDAId (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII & BamHI
Gene Size432 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YDAId (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) YdaI-F: gccgaagcttACTTGACCAGATTGATCT
Reverse Primer:(cgcggatcc tag) YdaI-R: cgcggatccTCTGTTACAGATGGAGGC
Length of the Cloned Region: 6 to 113 Length: 108
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDAId