Data for the Constructed Mutant: LRPCd
General Information
Name of the Mutant
LRPCd (disruption)
Host
168
Used Vector
pMutinT3
Used Enzyme Sites
HindIII and BamHI
Gene Size
432 (nt)
Growth & Expression (reporter lacZ):
in DSM:
no
in MM:
no
Mutant Submission to NIG:
Correspondence:
Mutant Construction for LRPCd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
gccgaagctt
tag)
lrpC-F:
gccgaagcttACTTGACCAGATTGATCT
Reverse Primer:
(
cgcggatcc
tag)
lrpC-R:
cgcggatccTCTGTTACAGATGGAGGC
Length of the Cloned Region: 6 to 113 Length: 108
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
LRPCd