Data for the Constructed Mutant: YDAEd

General Information

Name of the MutantYDAEd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size501 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:

Mutant Construction for YDAEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydaE-F: gccgaagcttCGAAAGAAGAAGTGAACAGC
Reverse Primer:(cgcggatcc tag) ydaE-R: cgcggatccTGGATGGCGGTGTTCCGG
Length of the Cloned Region: 11 to 216 Length: 206
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDAEd