Data for the Constructed Mutant: YDABd

General Information

Name of the MutantYDABd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1395 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YDABd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) ydaB-F: gccgaagcttCATGACATGCCGAATAGC
Reverse Primer:(cgcggatcc tag) ydaB-R: cgcggatccCTGCCAGCTGACAGGTAC
Length of the Cloned Region: 28 to 255 Length: 228
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YDABd