Data for the Constructed Mutant: YCNJd

General Information

Name of the MutantYCNJd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1623 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCNJd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycnJ1: gcgcaagcttTCTTCACCTGGGGAAAACAG
Reverse Primer:(gccgagatct tag) ycnJ2: gccgagatctATTCAGCGCTGAATCGGCTG
Length of the Cloned Region: 88 to 414 Length: 327
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCNJd