Data for the Constructed Mutant: YCNId

General Information

Name of the MutantYCNId (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size612 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCNId (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycnI1: gcgcaagcttGACACTATGTCCGGCTATTG
Reverse Primer:(gccgagatct tag) ycnI2: gccgagatctGGCAACAAAGGTAAACTGCT
Length of the Cloned Region: 21 to 336 Length: 316
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCNId