Data for the Constructed Mutant: YCNGd

General Information

Name of the MutantYCNGd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1308 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCNGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycnG1: gcgcaagcttCATCACAACTGCACAATGGC
Reverse Primer:(gccgagatct tag) ycnG2: gccgagatctCGCTTTTTTCTCATGGCTGC
Length of the Cloned Region: 21 to 333 Length: 313
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCNGd